recombinant human ctrp3 rhctrp3  (R&D Systems)

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: CTRP3 Regulates Endochondral Ossification and Bone Remodeling During Fracture Healing

doi: 10.1002/jor.24553

Figure Lengend Snippet: (A) Representative whole-femur μCT isosurface renderings at 5, 10 and 20dpf for CTRP3 KO, WT and TG mice. Femurs were scanned after removal of intramedullary pins. Apparent bends in 5dpf bones are artifacts of this removal: fractures are straight in vivo. Quantitative callus μCT data for (B) bone volume fraction (BV/TV), (C) bone mineral content (BMC) and (D) bone mineral density (BMD). Solid lines represent p<0.05 by genotype. Maroon up-arrows represent female mice and navy down-arrows represent male mice. For additional quantitative measures of calluses, see Fig S1.

Article Snippet: The fracture procedure was then repeated in WT mice aged 34±3 weeks to test a systemic protein delivery strategy; animals in this cohort received either phosphate buffered saline (PBS) vehicle (Vh) or 5.0μg recombinant human CTRP3 (rhCTRP3) (R&D Systems, produced in mouse NS0 cells) in PBS and harvested at 21dpf.

Techniques: In Vivo

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: CTRP3 Regulates Endochondral Ossification and Bone Remodeling During Fracture Healing

doi: 10.1002/jor.24553

Figure Lengend Snippet: Safranin-O micrographs from CTRP3 KO, WT and TG mice at (A) 5dpf, (B) 10dpf and (C) 20dpf. Images are oriented to show one side of the callus in longitudinal section with cortical bone at the bottom (light blue staining). Three selected areas of callus (white squares) are magnified on the right of each panel. For associated histomorphometry, see Fig S2.

Article Snippet: The fracture procedure was then repeated in WT mice aged 34±3 weeks to test a systemic protein delivery strategy; animals in this cohort received either phosphate buffered saline (PBS) vehicle (Vh) or 5.0μg recombinant human CTRP3 (rhCTRP3) (R&D Systems, produced in mouse NS0 cells) in PBS and harvested at 21dpf.

Techniques: Staining

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: CTRP3 Regulates Endochondral Ossification and Bone Remodeling During Fracture Healing

doi: 10.1002/jor.24553

Figure Lengend Snippet: Whole-callus mRNA expression of (A) CTRP3, (B) α-actin-2 (ACTA2), (C) osterix (SP7), (D) collagen-II (COL2A1) and (E) tartrate-resistant acid phosphatase (TRAP) vary based on CTRP3 genotype. Relative expression is presented at 2−dCt with ACTB as a housekeeping gene. Statistics were run on dCt values. Solid lines represent p<0.05 by genotype. Maroon up-arrows represent female mice and navy down-arrows represent male mice.

Article Snippet: The fracture procedure was then repeated in WT mice aged 34±3 weeks to test a systemic protein delivery strategy; animals in this cohort received either phosphate buffered saline (PBS) vehicle (Vh) or 5.0μg recombinant human CTRP3 (rhCTRP3) (R&D Systems, produced in mouse NS0 cells) in PBS and harvested at 21dpf.

Techniques: Expressing

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: CTRP3 Regulates Endochondral Ossification and Bone Remodeling During Fracture Healing

doi: 10.1002/jor.24553

Figure Lengend Snippet: μCT (A) quantitation and (B) isosurface renderings at 21dpf in mice that received either subcutaneous Vh or 5μg rhCTRP3 protein at time of surgery. Outputs include total volume (TV), bone volume (BV), bone volume fraction (BV/TV), bone mineral content (BMC), bone mineral density (BMD), tissue mineral content (TMC) and tissue mineral density (TMD). Maroon up-arrows represent female mice and navy down-arrows represent male mice.

Article Snippet: The fracture procedure was then repeated in WT mice aged 34±3 weeks to test a systemic protein delivery strategy; animals in this cohort received either phosphate buffered saline (PBS) vehicle (Vh) or 5.0μg recombinant human CTRP3 (rhCTRP3) (R&D Systems, produced in mouse NS0 cells) in PBS and harvested at 21dpf.

Techniques: Quantitation Assay

Journal: Redox Biology

Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

doi: 10.1016/j.redox.2018.07.007

Figure Lengend Snippet: Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.

Article Snippet: Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA).

Techniques: Expressing, Immunostaining

Journal: Redox Biology

Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

doi: 10.1016/j.redox.2018.07.007

Figure Lengend Snippet: Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

Article Snippet: Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA).

Techniques: Expressing, Immunostaining

Journal: Redox Biology

Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

doi: 10.1016/j.redox.2018.07.007

Figure Lengend Snippet: Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

Article Snippet: Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA).

Techniques:

Journal: Redox Biology

Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

doi: 10.1016/j.redox.2018.07.007

Figure Lengend Snippet: Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

Article Snippet: Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA).

Techniques: Derivative Assay, Expressing, Cell Culture, Immunostaining, Staining, TUNEL Assay, Recombinant

Journal: Redox Biology

Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction

doi: 10.1016/j.redox.2018.07.007

Figure Lengend Snippet: miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.

Article Snippet: Recombinant human CTRP3 (rhCTRP3) was purchased from Aviscera Bioscience (Santa Clara, CA, USA).

Techniques: Activation Assay, Recombinant